DNA Origami: folding on the smallest scale

DNA Origami: folding on the smallest scale

Thank you.

Thank you.

200:01:05 150 –> 00:01:05 479 That's amazing.

300:01:05 479 –> 00:01:09 300 Thank you.

Hey, why don't you take a seat? Thanks.

That is really fascinating.

Yes, it is wonderful how you can make somethingso complex out of something really simple.

really simple.

Yes, the possibilities areendless and the material is just everywhere.

Definitely.

Hey, do youfold as well? Actually I do fold.

I fold DNA.

DNA? I didn't know that was possible! Let me show you how.

DNA origami follows one simple rule.

Whenever two strands of DNA match theybind and form a double helix.

What if you want to make a crocodile? You use DNA that binds in different places.

And DNA origami is so simple.

You draw to shape you want on thecomputer the computer calculates the 1700:02:21 969 –> 00:02:23 359 sequences you need you email the sequences off to a companyand then you get such a little white box.

Each of the wells here contains one DNA sequence.

That sounds like something from sciencefiction.

But since 2006 it's been a 2200:02:42 150 –> 00:02:42 950 reality So, shall we make some origami then? Yes,let's.

Then we just need to mix.

I am now just pipetting DNA in water.

so each of the droplets now containsone DNA sequence.

We mix them with a pipette tip and put them in this tube and then we need to heat and the DNAorigami is ready If your paper origami model was the size of the actual DNA origami channelthen a grain of sand would be the size of Mount Everest.

Really? So you are a nanodesigner? Maybe.

But just like origami hasapplications in the real world like 3300:03:48 740 –> 00:03:51 200 folding airbags to make cars safer we're also working towards applications.

We're trying to make these small 3500:03:55 640 –> 00:04:00 20 channels from DNA origami.

We can make them change size and shapereacting to their environment.

We can make them punch holes intomembranes and we can even make them glow by attaching special molecules to DNA.

How do you know the DNA channels actuallypunch holes into membranes when they are too small to see? Good point.

It's very hard to observe a single channel 4100:04:24 310 –> 00:04:25 360 directly but we can observe their function.

Weseal this glass capillary with a membrane.

When a channel punches a hole into this membrane theseal is no longer perfect and we can observe a current going through.

Okay.

That's pretty cool.

But is it as cool as origami crocodiles? Many genetic diseases lead to defectivechannels in cells.

Now imagine what we could do if we couldcreate artificial channels for every patientthat way we need them.

With DNA origami we can build onthe nanoscale.

A versatile tool with versatileapplications from medicine to 5300:05:12 880 –> 00:05:14 530 miniaturized computers.

We may be curious to see what is yet tocome.

Source: Youtube